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BLOG STARTED: 10/18/2010
BLOG DIED: ??/??/??
current layout: science box
last updated: october/18/10
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HELLO TECHNOLOGISTS
welcome to our kawaii blog. feel free to read around our activities.
this blog was designed for our course IBG 302. this is for our lovely lecturer ;Puan Wan Nadiah
COPYCATS, rippers & spammers are not welcome here!
strictly NO RIPPING
US
Jack, Minx, Rat, Tiina, Salwa, Dilla.
third year students of BIOPROCESS TECHNOLOGY division,
UNIVERSITI SAINS MALAYSIA.
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 Saturday, October 23, 2010  The 1st day @ 11:06 PM Today, 04/10/2010 is the first day of our IBG 302 practical class. Special thanks to our School of Industrial Technology, Dr. Rosma, Puan Wan Nadiah, Lab assistants, graduate assistants for guiding us during this practical class and giving us this golden opportunity to have an in-depth look and understanding about the installation, utilization, and some precaution steps when dealing with the bioreactors. Not to miss out, we appreciate Mr. Shaman from Infors-HT for presenting the information about the bioreactor we are going to use in this practical class, from the very beginning to the end.
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| Mr.Shaman |
This is Mr. Shaman!! In the beginning of the practical class, he gave a little bit presentation about the history of the company, the organization, company’s philosophy and also the location.
First of all, allow me to introduce to you our Bioreactor, it is a 2.5L minifors bioreactor. And the title for our practical class is the fermentation process using yeast, S.cerevisiae. There are normally 3 parts of a bioreactor, instrumentation, control cabinet and the vessel. To ensure optimum fermentation process, the maximum volume of medium we can add into our fermentor is 1.8L.
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Parts of the Minifors (bioreactor) we used for this lab
The reason of using bioreactor instead of shake flask is due to some reasons. First, if we use shake flask, medium can be used up and this leads to death of cells. However, if bioreactor is being used, sterilized medium can be fed in and bioreactor can be used in large scale, up to few thousand liters. Bioreactor is also suitable for the production of vaccine, penicillin and some culture drinks like vitagen and yakult.
For fermentation process involved bacteria or yeast, acid is normally produced itself. Therefore, we do not need to add in acid buffer. Base buffer is used in this case.
After explaining and showing us how to install a bioreactor, we continue with the media preparation. The medium we are going to use consists of 1% yeast extract, 2% peptone and 2% glucose. Then, we added in 1.5L of distilled water into the medium and stirred it. Then, we put the medium and the inoculation flask into autoclave.
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6ix of us
Jack - the class leader! :DD
Tiina - the stunning Finnish girl :))
Minx - blog creator. ;)
Rat - the craziest. :P
Salwa - as sweet as her name. ;;)
Dilla - the brainiac. =B |
Saturday, October 23, 2010 The 1st day @ 11:06 PM Today, 04/10/2010 is the first day of our IBG 302 practical class. Special thanks to our School of Industrial Technology, Dr. Rosma, Puan Wan Nadiah, Lab assistants, graduate assistants for guiding us during this practical class and giving us this golden opportunity to have an in-depth look and understanding about the installation, utilization, and some precaution steps when dealing with the bioreactors. Not to miss out, we appreciate Mr. Shaman from Infors-HT for presenting the information about the bioreactor we are going to use in this practical class, from the very beginning to the end.
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| Mr.Shaman |
This is Mr. Shaman!! In the beginning of the practical class, he gave a little bit presentation about the history of the company, the organization, company’s philosophy and also the location.
First of all, allow me to introduce to you our Bioreactor, it is a 2.5L minifors bioreactor. And the title for our practical class is the fermentation process using yeast, S.cerevisiae. There are normally 3 parts of a bioreactor, instrumentation, control cabinet and the vessel. To ensure optimum fermentation process, the maximum volume of medium we can add into our fermentor is 1.8L.
|
Parts of the Minifors (bioreactor) we used for this lab
The reason of using bioreactor instead of shake flask is due to some reasons. First, if we use shake flask, medium can be used up and this leads to death of cells. However, if bioreactor is being used, sterilized medium can be fed in and bioreactor can be used in large scale, up to few thousand liters. Bioreactor is also suitable for the production of vaccine, penicillin and some culture drinks like vitagen and yakult.
For fermentation process involved bacteria or yeast, acid is normally produced itself. Therefore, we do not need to add in acid buffer. Base buffer is used in this case.
After explaining and showing us how to install a bioreactor, we continue with the media preparation. The medium we are going to use consists of 1% yeast extract, 2% peptone and 2% glucose. Then, we added in 1.5L of distilled water into the medium and stirred it. Then, we put the medium and the inoculation flask into autoclave.
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designer: stephanie
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affiliates
Those Close ones
 friend @ MINX
friend @ RAT
friend @ TIINA
friend @ SALWA
friend @ DILLA
friend @ JACK
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